Description
NuDT10, also known as APS2 or DIPP3alpha, is a member of the Nudix hydrolase family of pyrophosphatases. Nudix hydrolases contain a characteristic Nudix domain and are responsible for catalyzing the hydrolysis of nucleoside diphosphate derivatives. It functions as a manganese-dependent polyphosphate phosphohydrolase with an optimum pH of 8.5. This protein specifically metabolizes diadendosine-polyphosphates and, to a lesser extent, diphosphoinositol polyphosphates. Recombinant human NuDT10 protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography